
(Ms)Sunita Passi
Tri-Dosha Ltd.
Abstract Title: Evaluating the Potential of Neem in Hair Care: An In Vitro Cytotoxicity Study by Tri-Dosha and MTIF
Biography: Sunita Passi is an Ayurvedic Practitioner, entrepreneur, and advocate for holistic wellness. As the founder of Tri-Dosha, she leads the development of natural skincare rooted in Ayurvedic traditions, including research-driven formulations harnessing the power of botanicals like neem. Sunita is also a respected voice in the media, having hosted a BBC radio show and a podcast focused on integrative eastern wellness practices. Her work bridges traditional wisdom with modern science, bringing Ayurvedic solutions to a wider audience.
Research Interest: Tri Dosha Ltd has partnered with the Medical Technologies Innovation Facility (MTIF) to investigate the health benefits of Neem-based products, particularly in hair care formulations. This study aims to evaluate the potential of Neem in enhancing scalp health, focusing on its inclusion in shampoos and conditioners. Initial discussions between Tri Dosha Ltd and MTIF researchers established specific objectives, which led to the development of a structured research proposal. The study involves an in vitro cytotoxicity assessment to determine how human cells respond to Neem-based formulations. MTIF's cutting-edge facilities and expert team are well-equipped to carry out this research, providing high-quality results without the need for Tri Dosha Ltd to invest in costly equipment. Over five days, MTIF will treat normal human skin cells—both keratinocytes and fibroblasts—with Neem shampoo, conditioner, and balm. Cytotoxicity will be evaluated using the luminescence-based CellTiter-Glo® 2.0 assay, assessing cell viability under controlled conditions. The study design includes untreated cells and positive controls to ensure the accuracy of results. This study will generate valuable insights into the biological impact of Neem on scalp health, though it is important to note that the scope is limited to in vitro analysis and does not imply clinical efficacy. The findings will be documented in a comprehensive report, which will aid Tri Dosha Ltd in determining the next steps for product development. This collaboration highlights the potential of scientific research in validating traditional medicine claims, with Neem's rich history in Ayurvedic healing being rigorously tested in modern cosmetic applications.